PURIFICATION AND SOME PROPERTIES OF CELLULASE FROM ODONTOTERMES FORMOSANUS （ISOPTERA： TERMITIDAE）

编号 zgly0000331419

文献类型 期刊论文

文献题名 PURIFICATION AND SOME PROPERTIES OF CELLULASE FROM ODONTOTERMES FORMOSANUS （ISOPTERA： TERMITIDAE）

学科分类 220.3020;森林昆虫学

作者 Tian-ciYang  Jian-chuMo  Jia-anCheng 

作者单位 UrbanEntomologyResearchCenter 

母体文献 Entomologia Sinica（中国昆虫科学：英文版） 

年卷期 2004,11(1)

页码 1-10

年份 2004 

分类号 Q969.296 

关键词 黑翅土白蚁  纯化  中纤维素酶  内切葡聚糖酶  双向电泳 

文摘内容 The purification of the cellulase from Odontotermes forrnosanus workers was achieved by using anion-exchange column of UNOsphere Q, BioLogic DuoFlow chromatography system. The purified cellulase was identified as an endoglucanase and some of its properties were investigated. The EGase activity was 807.5-fold as high as the initial enzyme activity using CMC as substrate and 14.4-fold using salicin as substrate. The enzyme preparations were homogeneous as judged by SDS-PAGE electrophoresis, molecular weight of which was 80 kDa and confirmed by 2-DE zymogram analysis. The enzyme was isoelectric at pH 6.4, which was active on CMC substrate。