Genetic transformation of crylA (b) gene into teak

编号 zgly0000303351

文献类型 期刊论文

文献题名 Genetic transformation of crylA (b) gene into teak

学科分类 220.30;森林保护学

作者 NorwatiA  AbdullahR  MohdRosliH  NorliaB. 

作者单位 ForestResearchInstituteMalaysia(FRIM 

母体文献 分子植物育种 

年卷期 2003,1(1)

页码 53-57

年份 2003 

分类号 S763.729.9 

关键词 柚木  基因转化  crylA(b)基因  害虫综合治理  基因重组  PCR  潮霉素 

文摘内容 Teak (Tectona grandis) provides one of the most highly sought after timber in the world and is a widely recommended species for reforestation. As such teak is widely planted in Malaysia. Though no serious outbreaks have been recorded for teak in Malaysia, but insect attack remains the most important threat to the timber industry. Thus, in efforts to overcome the problem, an integrated pest management system needs to be developed. Spraying of commercial Bt has been a common practice in addressing minor outbreaks. However, one of the main limitations of the spraying technique is poor coverage, especially on plant surfaces. Poor coverage, however, could be overcome by planting insect resistant trees. In addition, the approach ot using genetic engineering in addressing the above problem proves to be possible with the advancement made in genetic transformation of trees especially in the last decade. This, together with improved knowledge on gene function following improved DNA recombinant techniques promises the major advancement in pest management of forest species. This report demonstrates the possibility of transferring foreign gene into teak cells. In this study, nodal segments of teak were subjected to particle bombardment. Nodal segments bombarded with gold particles coated with plasmid DNA carrying hygromycin phosphotransferase ( hpt ), fl-glucuronidase (flus) and crylA (b) genes were then transferred onto medium for shoot development. The shoots were than transferred onto the same medium supplemented with 10mg/L hygromycin for selection. Selection was repeated several times with six-week subculture intervals on the same Hm-containing media. The presence of the transgenes in the Hmr plants was confirmed using PCR。