cDNA cloning and functional analysis of 4-coumarate——CoA. ligase (4CL) gene in Chinese white aspen

编号 zgly0000302712

文献类型 期刊论文

文献题名 cDNA cloning and functional analysis of 4-coumarate——CoA. ligase (4CL) gene in Chinese white aspen

学科分类 220.99;林学其他学科

作者 ZHAOHuayan  WEIJianhua  LUJing  SONGYanru  WANGTai  ZHUZhiqing 

作者单位 KeyLaboratoryofPhotosynthesisandEnvironmentalMolecularPhysiology 

母体文献 Progress in Natural Science;自然科学进展: 英文版 

年卷期 2003,13(12)

页码 895-900

年份 2003 

分类号 S792.117  Q785 

关键词 毛白杨  4CL基因  连接酶基因  基因克隆  泛函分析  RT-PCR  生物技术 

文摘内容 cDNA encoding 4-eoumarate: CoA ligase (4CL) was isolated from the secondary developing xylem of Chinese white aspen (Populus tomentosa ) by RT-PCR for the first time, which was 1619 bp in length. The coding sequence and putative amino acid sequence showed 97.53 % and 97.00 % identity to that of Pt4CL1 in quaking aspen (P. tremuloids ), respectively. Molecular analysis indicated that 4CL was encoded by multiple genes in P. tomentosa, its mRNA was highly accumulated in xylem and the expression of 4CL revealed a biphasie pattern in one growing season, almost in phase with the expression of other related enzymes in lignin biosynthesis.Transgenie research showed that expression of antisense 4CL eDNA led to the decreasing of lignin content in transgenie tobaccos, among which the average reduction was 10.3 % and the highest could be up to 18.9 %. These data suggested that 4CL gene was a potential gene used in altering lignin biosynthesis by biotechnology for producing new materials of papermaking。