Identification of Taxus cuspidata Sieb. et Zucc. endophytic fungi-new species, species known and their metabolite

编号 zgly0000301515

文献类型 期刊论文

文献题名 Identification of Taxus cuspidata Sieb. et Zucc. endophytic fungi-new species, species known and their metabolite

学科分类 220.10;林业基础学科

作者 XlANGYong  LUAn-guo  WUWen-fang 

作者单位 ShenyangPharmaceuticalUniversity 

母体文献 Journal of Forestry Research;林业研究: 英文版 

年卷期 2003,14(4)

页码 290-294

年份 2003 

分类号 S718.81  Q949.32 

关键词 真菌  树皮  紫杉  长白山自然保护区  原始森林  种  分离  鉴定  变异  发酵  代谢物  菌株 

文摘内容 A total of 94 isolates of endophytic fungi were isolated from the bark of 200-yr.-old Taxus cuspidata Sieb. et Zucc.in the primeval forest of the Changbai Mountain Natural Reserve, and 19 species of endophytic fungi were identified, including 10 new recorded-genus-species, 2 new species (Phomopsis Iongiscoleosporu Y. Xiang et Lu An Guo Wu Wen Fang, Coniothyrium macrospoum Y. Xiang J.X. et Lu An Guo Wu Wen Fang), 1 new varied species (Alternaria alternata (Fr.) Keissler var. taxi Y.Xiang et Lu An Guo) and 6 known species of China (Eurotium amstelodomi Mgngin, Eurotium repens de Bary, Botrytis sp.,Penicillium citrinum Thom, Epicoccum nigrium LinK, Fusarium sp.). Through thin layer chromatography (TLC), liquid fermentation metabolite of the strains was determined, and four strains (Alternaria alternata (Fr.) Keissler var. taxi Y. Xiang et Lu An Guo Wu Wen Fang, Botrytis sp., Eurotium amsteloodomi Mgngin, Eurotium repens de Bary) were screened out, whose metabolites reacted positively with the vanillic aldehyde that was one special taxoid developer. Among the four strains, Alternaria alternata (Fr.) Keissler var. taxi Y. Xiang et Lu An Guo, produced one compound largely, which positively reacted with one alkaloids developer-Bismuth potassium iodide. The compound is identified as taxoids type through spectrum analysis. This demonstrates that Alternaria alternata (Fr.) Keissler var. taxiY. Xiang et Lu An Guo can highly produce taxoids largely。