银杏胚轴、子叶诱导胚状体发生及成苗的研究

编号 zgly0000370160

文献类型 期刊论文

文献题名 银杏胚轴、子叶诱导胚状体发生及成苗的研究

作者 郭长禄  陈力耕  何新华  SyedAsgarTori  袁海英 

作者单位 浙江大学农业部园艺植物生长发育与生物技术重点开放实验室  浙江大学农业部园艺植物生长发育与生物技术重点开放实验室  浙江大学农业部园艺植物生长发育与生物技术重点开放实验室 

母体文献 林业科学 

年卷期 2005,41(2)

页码 178-181,F003

年份 2005 

分类号 S666.036  Q949.761.7 

关键词 胚状体发生  胚轴  银杏  成苗  诱导  子叶  axis  6-BA  best  and  was  for  NAA  the  mg  The  pla 

文摘内容 Embryonic axis and cotyledon of immature and mature embryos were induced as explants for embryogenesis in Ginkgo biloba. Results showed somatic embryos could be induced only through embryonic axis and cotyledon of immature embryos. MK + NAA 1 mg·L^-1 + 6-BA 1 mg·L^-1 was the best medium for somatic embryogenesis from embryonic axis, and somatic embryogenesis rate was 45.28%. MK + NAA 1.5 mg·L^-1 + 6-BA 1 mg·L^-1 was the best medium for somatic embryogenesis from cotyledon, and somatic embryogenesis rate was 12.9%. The growth and development of somatic embryos were promoted by adding 10% coconut milk into MK, and 34.48% somatic embryos could develop into plants。