Changes of plasma membrane ATPase activity, membrane potential and transmembrane proton gradient in Kandelia candel and Avicennia marina seedlings with various salinities

编号 zgly0000350049

文献类型 期刊论文

文献题名 Changes of plasma membrane ATPase activity, membrane potential and transmembrane proton gradient in Kandelia candel and Avicennia marina seedlings with various salinities

学科分类 220.10;林业基础学科

作者 ZHAOZhong-qiu  ZHENGHai-lei  ZHUYong-guan 

作者单位 ResearchCenterforEco-EnvironmentalSciences  SchoolofLifeSciences 

母体文献 Journal of Environmental Sciences;环境科学学报: 英文版 

年卷期 2004,16(5)

页码 742-745

年份 2004 

分类号 X173 

关键词 质膜  ATP酶  活性  树苗  盐度  环境保护  植物 

文摘内容 The salt-secreting mangrove, Avicennia marina, and non-salt-secreting mangrove, Kande/ia candel were cultivated in sand with various salinities(0‰, 100‰, 20‰, 30‰, 40‰) for 60 d. Plasma membrane vesicles of highpurity in leaves and roots of A. marina and K. candel seedlings were obtained by two-phase partitioning. The function of the plasma membranes, the activity of ATPase, membrane potential and transmembrane proton gradient, at various salinities were investigated. The results showed that within a certain range of salinity( A. marina and roots of K. candel: 0-30‰; leaves of K. candel: 0-20‰), the activity of ATPase increased with increasing salinity, while high salinity (above 30‰ or 20‰) inhibited ATPase activity. In comparison with A. marina, K.candel appeared to be more sensitive to salinity. The dynamics of membrane potential and transmembrane proton gradient in leaves and roots of A. marina and K. candel seedlings were similar to that of ATPase. When treated directly by NaCl all the indexes were inhibited markedly: there was a little increase within 0-10‰( K. candel) or 0-20‰( A. marina) followed by sharp declining. It indicated that the structure and function of plasma membrane was damaged severely。