One Rapid and Efficient Method for Isolation of Total RNA from Shoots Regenerated in vitro of Populus suaveolens

编号 zgly0000327892

文献类型 期刊论文

文献题名 One Rapid and Efficient Method for Isolation of Total RNA from Shoots Regenerated in vitro of Populus suaveolens

学科分类 220.99;林学其他学科

作者 LinYuanzhen  LinShanzhi  ZhangZhiyi  HeChengzhong  GuoHuan  ZhangWei 

作者单位 KeyLaboratoryforGeneticsandBreedinginForestTreesandOrnamentalPlants 

母体文献 中国林学: 英文版 

年卷期 2004,6(1)

页码 18-21

年份 2004 

分类号 S792.110.1 

关键词 杨树  植株再生  体外培养  RNA  分离  rRNA  CTAB 

文摘内容 Total RNA was isolated from shoots regenerated in vitro of Populus suaveolens by the modified method of CTAB,and two clear bands of rRNA (28S and 18S) were observed in agarose electrophoresis. In addition, the values of OD260/OD280 and OD260/OD230 of extracted RNA were 2.12 and 2.23 respectively. The results show that RNA is little decomposed and the purity of RNA is high. Moreover, RNA isolated by the modified method of CTAB reagent had been successfully used for reverse transcription of P.suaveolens cDNAs and ideal special band was observed。